SUMO Protease
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SUMO 蛋白酶，也叫 Ulp，是一种源于酿酒酵母 Ulp1（Ubl-specific protease 1）重组基因片段的高活性半胱氨酸蛋白酶。SUMO 蛋白酶可以高度特异性的方式识别和切割泛素样（UBL）蛋白 SUMO 羧基端(C端) Gly-Gly 后的肽键。SUMO 是一种泛素样蛋白(Ubiquitin- like Protein)，常见于蛋白翻译后修饰(post-translation modification, PTM)，对于蛋白的稳定性、生物学功能的调节有重要作用。
SUMO 蛋白酶的最佳解裂温度为 30 ºC，最优 pH 为 8.0，但其在较宽的 pH 范围(6.0-10.0)、温度范围(2~30℃)、离子强度范围(0-400mM NaCl)内均保持有较高的酶活性。在特殊场景使用中，可在4℃温度下对样品进行酶切过夜来保持目的蛋白的结构和生物活性。SUMO Protease 在还原剂 DTT(0.5~2mM)存在下的酶切活性更高，酶切体系中加入适当浓度DTT 可以显着提高酶切效率，尤其是在长时间的酶切过程中，例如 4ºC 酶切过夜。裂解后的SUMO蛋白酶可利用其 N 末端的组氨酸标签进行亲和层析去除。
| Components | Detailed composition | Specification | ||
| 200U | 1000U | 5000U | ||
| SUMO Protease (10U/µl) | 25 mM Tris-HCl, pH 8.0 0.1% Igepal (NP-40) 250 mM NaCl 500 μM DTT 50% (v/v) glycerol |
20 µl | 100 µl | 500 µl |
| 10X SUMO Protease Buffer + Salt | 500 mM Tris acetate, pH 8.0 2% Igepal (NP-40) 1.5 M NaCl 10 mM DTT |
400 µl | 2×1 ml | 10×1 ml |
| 10X SUMO Protease Buffer – Salt | 500 mM Tris acetate, pH 8.0 2% Igepal (NP-40) 10 mM DTT |
400 µl | 2×1 ml | 10×1 ml |
Long-term storage: -80 °C | ||||