T3 RNA Polymerase (RNase-free)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
噬菌体T3 RNA Polymerase分子量约99 kDa，是一种DNA依赖的5’-3’ RNA聚合酶，对T3噬菌体启动子（5’-AATTAACCCTCACTAAAGGGAGA-3’）具有高度特异性。该酶通过催化单链或双链DNA T3启动子下游NTP的掺入，合成与T3启动子下游模板DNA互补的RNA链。
T3 RNA Polymerase可识别包含修饰核苷酸如生物素、荧光素、地高辛标记在内的NTP底物，催化产生的RNA在生物学实验有许多应用，常见于制备RNA杂交探针，基因组DNA序列分析，核糖核酸酶保护测定（RNase protection assay），反义RNA、RNAi合成，体外翻译以及RNA结构和功能等相关研究。
| Components | 2000 U | 5000 U | 10000 U |
| T3 RNA Polymerase (50 U/μL) | 0.04 mL | 0.1 mL | 0.2 mL |
| 10X T3 RNA Polymerase Reaction Buffer | 0.2 mL | 0.5 mL | 1 mL |
Store the components at -20 °C. | |||