SP6 RNA Polymerase
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SP6 RNA Polymerase是一种高度特异识别SP6启动子序列的DNA依赖的5'→3' RNA聚合酶。SP6 RNA聚合酶可以催化单链或双链DNA SP6启动子下游NTP的掺入，合成与SP6启动子下游的模板DNA互补的RNA。SP6 promoter sequence为ATTTAGGTGACACTATAGAA，其中最后一个G为转录的第一个碱基。
SP6 RNA Polymerase合成的RNA可以用于杂交探针、基因组DNA序列分析、核糖核酸酶保护测定(RNase protection assay)、反义RNA合成、作为体外翻译的RNA模板、RNA剪接研究的底物、RNA二级结构和RNA-蛋白质相互作用、核酸扩增分析、siRNA、miRNA等小RNA等方面。
| Components | 2000 U | 5000 U | 10000 U |
| SP6 RNA Polymerase (20U/μl) | 0.1 ml | 0.25 ml | 0.5 ml |
| 10X SP6 Reaction Buffer | 0.2 ml | 0.5 ml | 1 ml |
Store at -20 °C. | |||