SR 3576
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | Off White solid |
| Storage | Store at -20°C |
| M.Wt | 501.53 |
| Cas No. | 1164153-22-3 |
| Formula | C27H27N5O5 |
| Solubility | <50.15mg/ml in DMSO |
| Chemical Name | 3-(4-(3-(m-tolyl)ureido)-1H-pyrazol-1-yl)-N-(3,4,5-trimethoxyphenyl)benzamide |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC(N2N=CC(NC(NC3=CC=CC(C)=C3)=O)=C2)=CC=C1)NC(C=C4OC)=CC(OC)=C4OC |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]： | |
|
细胞系 |
人卵巢癌cisplatin抗性SKOV3/DDP细胞 |
|
溶解方法 |
在DMSO中的溶解度大于10 mM。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
24 h，2 μM |
|
应用 |
SR 3576是JNK3的特异性抑制剂。在人卵巢癌SKOV3/DDP细胞中，使用SR 3576抑制JNK3的活性可加重氧化应激，抑制自噬流，从而导致S1诱导的凋亡增加。 |
|
References: [1]. Yang X, Xiang X, Xia M, et al. Inhibition of JNK3 promotes apoptosis induced by BH3 mimetic S1 in chemoresistant human ovarian cancer cells[J]. The Anatomical Record, 2015, 298(2): 386-395. | |
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
相关生物数据
