Dimethylhydrazine dihydrochloride

Dimethylhydrazine dihydrochloride（CAS 号 306-37-6，简称 DMH）的核心生物学功能为诱导实验性结肠癌，主要通过引发 DNA 损伤（增加嘌呤 / 嘧啶缺失位点）、扰乱细胞周期调控（升高 p53、cyclin D1 表达）及肠道免疫微环境（改变 CD8⁺ T 细胞、树突状细胞（DCs）数量，调控 IFN-γ、穿孔素、颗粒酶等细胞因子 / 细胞毒性颗粒表达）发挥致癌作用；动物造模常见应用类型为大鼠实验性结肠癌模型（Wistar 大鼠为主），通过诱导结肠黏膜多发性斑块病变（MPLs）模拟癌变进程；常用应用浓度：动物实验中采用每周腹腔注射 20 mg/kg，持续 4 周，分为启动期（最后一次注射后 48 小时）和启动后期（处理后 12-16 周）两个观察阶段；其作为致癌物用于疾病模型构建，核心用途为评估潜在 chemopreventive 药物的抗肿瘤效果。

参考文献：

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