ML347
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ML347是有效的骨形态发生蛋白（BMP）受体选择性抑制，对ALK2的IC50值为32 nM[1]。
据发现，ML347是BMP I型受体ALK2的选择性抑制剂，并被用作探针分子。在体外激酶试验中，ML347有效抑制ALK1和ALK2，其IC50值分别为46 nM和32 nM。ML347对ALK3的IC50值超过10 μM，这表明ML347对ALK2的抑制作用比对ALK3的高出300倍。ML347对其它相关激酶（如ALK6和KDR）没有抑制作用。此外，在基于BMP4的细胞试验中，使用了C2C12BRA细胞，ML347同样显示出有效的抑制作用，其IC50值为152 nM[1]。
参考文献：
[1] Engers D W, Frist A Y, Lindsley C W, et al. Synthesis and structure–activity relationships of a novel and selective bone morphogenetic protein receptor (BMP) inhibitor derived from the pyrazolo [1.5-a] pyrimidine scaffold of Dorsomorphin: The discovery of ML347 as an ALK2 versus ALK3 selective MLPCN probe. Bioorganic & medicinal chemistry letters, 2013, 23(11): 3248-3252.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 352.39 |
| Cas No. | 1062368-49-3 |
| Formula | C22H16N4O |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥5.87 mg/mL in DMSO with gentle warming |
| SDF | Download SDF |
| Canonical SMILES | COC1=CC=C(C=C1)C2=CN3C(=C(C=N3)C4=C5C=CC=NC5=CC=C4)N=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | ML347是BMP受体的选择性抑制剂，对ALK2的IC50值为32 nM。 | |||||
| 靶点 | ALK2 | ALK1 | ||||
| IC50 | 32 nM | 46 nM | ||||
质量控制和MSDS
- 批次:
化学结构
