LDN-212854
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
LDN-212854是骨形态发生蛋白(BMP)信号通路的选择性抑制剂,IC50值为1.2 nM [1].
在激酶试验中,LDN-212854对caALK2和caALK5具有抑制活性,IC50值分别为16 nM和2 μM.有研究表明,与TGF-β信号相比,LDN-212854对BMP信号具有选择性.此外,LDN-212854对caALK2的抑制活性要比对caALK1和caALK3分别高6和10倍.在转染BMP受体的C2C12细胞中,LDN-212854也优先抑制caALK2.而且,在异位骨化的转基因ALK2Q207D模型中,LDN-212854处理能抑制异位骨形成,保持运动的肢体范围[1].
参考文献：
[1] Mohedas A H, Xing X, Armstrong K A, et al. Development of an ALK2-biased BMP type I receptor kinase inhibitor. ACS chemical biology, 2013, 8(6): 1291-1302.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 406.48 |
| Cas No. | 1432597-26-6 |
| Formula | C25H22N6 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥20.3 mg/mL in DMSO |
| SDF | Download SDF |
| Canonical SMILES | C1CN(CCN1)C2=CC=C(C=C2)C3=CN4C(=C(C=N4)C5=C6C=CC=NC6=CC=C5)N=C3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | LDN-212854是高效选择性BMP受体抑制剂,其对ALK2的IC50值为1.3 nM. | |||||
| 靶点 | ALK2 | |||||
| IC50 | 1.3 nM | |||||
质量控制和MSDS
- 批次:
化学结构
