WYE-687
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
WYE-687是一种ATP竞争性的mTOR抑制剂,IC50值为7 nM[1].
WYE-687是一种小分子的吡唑并嘧啶类mTOR1和mTOR2抑制剂.在使用His6-AKT和His6-S6K作为mTOR1和mTOR2特异性底物的免疫复合体激酶试验中,WYE-687剂量依赖性地阻止mTOR磷酸化其底物.此外,与PI3Kα(>100倍)\PI3Kγ(>500倍)以及其他24种蛋白激酶相比,WYE-687对mTOR具有高选择性.WYE-687在包括MDA361和HCT116在内的肿瘤细胞系中可引发强有力的G1期细胞周期停滞,抑制细胞生长.WYE-687也可影响癌细胞的血管生成因子.该物质可降低U87MG\MDA361和LNCap细胞中的HIF-1α表达[1].
参考文献：
[1] Yu K, Toral-Barza L, Shi C, et al. Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin. Cancer research, 2009, 69(15): 6232-6240.
| Storage | Store at -20°C |
| M.Wt | 528.61 |
| Cas No. | 1062161-90-3 |
| Formula | C28H32N8O3 |
| Solubility | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
| Chemical Name | methyl N-[4-[4-morpholin-4-yl-1-[1-(pyridin-3-ylmethyl)piperidin-4-yl]pyrazolo[3,4-d]pyrimidin-6-yl]phenyl]carbamate |
| SDF | Download SDF |
| Canonical SMILES | COC(=O)NC1=CC=C(C=C1)C2=NC3=C(C=NN3C4CCN(CC4)CC5=CN=CC=C5)C(=N2)N6CCOCC6 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | WYE-687是一种ATP竞争性的mTOR选择性抑制剂,IC50值为7 nM. | |||||
| 靶点 | mTOR | PI3Kα | PI3Kγ | p38α | Src | |
| IC50 | 7 nM | 0.81 μM | 3.11 μM | 28.9μM | >50 μM | |
化学结构
