Vialinin A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Vialinin A是泛素特异性肽酶（USP）的一个有效的抑制剂，对USP4、USP5/异肽酶T(IsoT)和泛素C末端水解酶L1去泛素化酶（UCH-L1 DUB）的IC50值分别为1.5 μM、5.9 μM和22.3 μM[1]。
USPs是去泛素化蛋白的一系列蛋白酶。泛素蛋白的修饰作用可以改变它在细胞中的功能、定位和命运。泛素水解酶在免疫和感染中起着重要的作用[2]。
在RBL-2H3细胞中，Vialinin A抑制肿瘤坏死因子（TNF-ɑ）的产生和释放，并抑制USP5的酶活性。在USP5 siRNA-敲除RBL-2H3细胞中，Vialinin A以剂量依赖的方式抑制TNF-ɑ的产生和释放，TNF-ɑ的mRNA表达量也显著降低[2]。在RBL-2H3细胞中，Vialinin A抑制TNF-ɑ的产生的IC50值为0.09 nM。Vialinin A也可抑制白介素4、已糖氨酶和单核细胞趋化蛋白1的释放，表明它具有抗过敏活性[3]。
参考文献：
[1]. Okada K, Ye YQ, Taniguchi K, et al. Vialinin A is a ubiquitin-specific peptidase inhibitor. Bioorg Med Chem Lett, 2013, 23(15): 4328-4331.
[2]. Yoshioka Y, Ye YQ, Okada K, et al. Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells. PLoS One, 2013, 8(12): e80931.
[3]. Onose J, Xie C, Ye YQ, et al. Vialinin A, a novel potent inhibitor of TNF-alpha production from RBL-2H3 cells. Biol Pharm Bull, 2008, 31(5): 831-833.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 562.57 |
| Cas No. | 858134-23-3 |
| Formula | C34H26O8 |
| Solubility | Soluble in DMSO |
| Chemical Name | [3,4-dihydroxy-2,5-bis(4-hydroxyphenyl)-6-(2-phenylacetyl)oxyphenyl] 2-phenylacetate |
| SDF | Download SDF |
| Canonical SMILES | C1=CC=C(C=C1)CC(=O)OC2=C(C(=C(C(=C2C3=CC=C(C=C3)O)O)O)C4=CC=C(C=C4)O)OC(=O)CC5=CC=CC=C5 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
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化学结构
