TH588
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TH588是MTH1的有效选择性抑制剂,IC50为5 nM.
MTH1(人Mutt同系物1)是抗突变嘌呤核苷三磷酸酶,用于审查氧化的核苷酸库.MTH1是一个新的癌症治疗靶标,可以防止癌细胞发生DNA损伤,如氧化应激诱导的细胞功能障碍或细胞死亡.
在U2OS和其它癌细胞系中,TH588选择性地有效杀死癌细胞,对一些原代或永生化的细胞毒性较少.TH588处理U2OS细胞,DNA的8-oxodG增加.在U2OS细胞中,MTH1抑制剂TH588诱导DNA损伤,激活ATM-p53介导的死亡应答和DNA修复[1].
BRAF V600E突变黑色素瘤异种移植小鼠对卡铂\达卡巴嗪和vemurafenib具有耐受性.十个肿瘤小鼠随机地每天一次接受TH588或对照组治疗.TH588治疗的小鼠肿瘤生长速度降低,尽管在24小时处理过程中,TH588的血清量下降到低于IC50浓度[1].
参考文献：
1. Gad H, Koolmeister T, Jemth AS et al. MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature. 2014 Apr 10;508(7495):215-21.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 295.17 |
| Cas No. | 1609960-31-7 |
| Formula | C13H12Cl2N4 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥7.38 mg/mL in DMSO |
| Chemical Name | (Z)-N-(6-(2,3-dichlorophenyl)-2-imino-2,3-dihydropyrimidin-4(1H)-ylidene)cyclopropanamine |
| SDF | Download SDF |
| Canonical SMILES | ClC1=CC=CC(C(NC2=N)=C/C(N2)=N/C3CC3)=C1Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
