Cy3 azide
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 5mg | ￥7500.00 ￥5250.00 | 现货 | |
| 25mg | ￥17500.00 ￥12250.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].
Reference:
[1] Sun, L. Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide–alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072–17075.
产品性质
| 分子式 | C34H44N6O7S2 |
| 分子量 | 712.88 |
| 化学名称 | 1-(6-((3-azidopropyl)amino)-6-oxohexyl)-2-((E)-3-((E)-1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| 溶解度 | ≥10 mg/mL in DMSO; ≥16.67 mg/mL in EtOH; ≥16.67 mg/mL in H2O |
| SMILES | O=C(NCCCN=[N+]=[N-])CCCCC[N+]1=C(/C=C/C=C2C(C)(C)C3=CC(S(=O)(O)=O)=CC=C3N\2CC)C(C)(C)C4=CC(S(=O)([O-])=O)=CC=C41 |
| 存储条件 | -20°C干燥避光 |
| 运输条件 | 蓝冰 |
| 最大发射波长 | 584nm |
| 最大激发波长 | 563nm |
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