Cy5.5 NHS ester (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cyanine5.5 NHS ester是用于标记含有伯胺的多肽,蛋白和寡核苷酸的反应性染料。 Cy5.5-NHS ester是一种近红外荧光染料,能够溶解在DMF和DMSO中,最大激发光和最大发射光分别在675 nm和693 nm左右[1]。该试剂不仅用于标记含有伯基的分子,而且还用于标记质粒DNA [2]。在生物分子标记中,该标记试剂的水溶性较低,先使用有机溶剂溶解该分子对于高效标记是必需的。首先,Cyanine染料应该溶解在有机溶剂中,然后加入包含生物分子的合适缓冲水溶液中。
通过皮下移植肿瘤小鼠的光学成像,在0.5nmol Cy5.5-NHS注射后30min至24h,皮下U87MG肿瘤可与周围背景组织明显区分开来。 Cy5.5-NHS的肿瘤吸收在注射后30分钟达到最大值,随着时间的推移缓慢洗出[3]。
References:
[1]. Ju Hee Ryu, Jin Hee Na, Ho Kyung Ko, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials, 2014, 35: 2302-2311.
[2]. Laura-Lee Farrell, Joel Pepin, Cezary Kucharski, et al. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC). European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 388–397.
[3]. Zhen Cheng, Jelena Levi, Zhengming Xiong, et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem., 2006, 17(3):662-669.
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Physical Appearance | A solid |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. 该产品在溶液中不稳定,建议现配现用 |
M.Wt | 716.31 |
Formula | C44H46ClN3O4 |
Solubility | ≥35.82 mg/mL in DMSO |
Chemical Name | 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium |
SDF | Download SDF |
Canonical SMILES | CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(ON4C(CCC4=O)=O)=O)=C1/C=C/C=C/C=C5C(C)(C)C6=C(C=CC=C7)C7=CC=C6N\5C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 684 | 最大发射光波 (nm) | 710 |
消光系数 (M-1cm-1) | 209000 | 量子产量 | 0.2 |
CF260 | 0.07 | CF280 | 0.03 |
质量控制和MSDS
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