Catalog No. A8103

Cy5.5 NHS ester (non-sulfonated)

Name: Cy5.5 NHS ester (non-sulfonated), Cy5.5 NHS酯（非磺化）, 1469277-96-0
Brand: APExBIO
SKU: A8103
Price: 2275 CNY
Availability: InStock

（别名：Cy5.5 NHS酯（非磺化））

氨基反应性荧光染料，不溶于水

规格	价格	货期	数量
5mg	￥2275.00	现货
25mg	￥4441.00	现货

CAS号：1469277-96-0纯度：98.00%

仅用于科研，不用于诊疗。未经明确授权不得转售。

021-55669583sales@apexbio.cnAPExBIO

特色产品

Annexin V-FITC/PI Apoptosis Kit

用于双染检测细胞凋亡和坏死

- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞

Phos binding reagent (Phosbind) acrylamide

分离和检测磷酸化/非磷酸化蛋白

- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白

Cell Counting Kit-8 (CCK-8)

用于细胞增殖和细胞毒性试验

- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂

Cy5 TSA Fluorescence System Kit

Cy5荧光标记的酪胺信号放大系统

- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率

EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

用于观察mRNA运送，定位，翻译

- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰

HotStart™ 2X Green qPCR Master Mix

用于荧光定量目标DNA或cDNA

- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单

HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus

高效的Cy5修饰RNA转录试剂盒

- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述产品性质生物相关数据质量控制操作说明产品引用文献摩尔浓度计算器

产品描述

Cyanine5.5 NHS ester是用于标记含有伯胺的多肽，蛋白和寡核苷酸的反应性染料。 Cy5.5-NHS ester是一种近红外荧光染料，能够溶解在DMF和DMSO中，最大激发光和最大发射光分别在675 nm和693 nm左右[1]。该试剂不仅用于标记含有伯基的分子，而且还用于标记质粒DNA [2]。在生物分子标记中，该标记试剂的水溶性较低，先使用有机溶剂溶解该分子对于高效标记是必需的。首先，Cyanine染料应该溶解在有机溶剂中，然后加入包含生物分子的合适缓冲水溶液中。

通过皮下移植肿瘤小鼠的光学成像，在0.5nmol Cy5.5-NHS注射后30min至24h，皮下U87MG肿瘤可与周围背景组织明显区分开来。 Cy5.5-NHS的肿瘤吸收在注射后30分钟达到最大值，随着时间的推移缓慢洗出[3]。

References:

[1]. Ju Hee Ryu, Jin Hee Na, Ho Kyung Ko, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials, 2014, 35: 2302-2311.
[2]. Laura-Lee Farrell, Joel Pepin, Cezary Kucharski, et al. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC). European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 388–397.
[3]. Zhen Cheng, Jelena Levi, Zhengming Xiong, et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem., 2006, 17(3):662-669.

产品性质

产品有效期	6个月
物理外观	A solid
CAS号	1469277-96-0
分子式	C₄₄H₄₆ClN₃O₄
分子量	716.31
化学名称	3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1,3-dihydro-2H-benzo[e]indol-2-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium chloride
溶解度	≥35.82 mg/mL in DMSO
SMILES	CC1(C)C(/C=C/C=C/C=C2C(C)(C)C(C3=CC=CC=C3C=C4)=C4N\2C)=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C6=C1C7=CC=CC=C7C=C6.[Cl-]
存储条件	-20°C干燥避光
运输条件	蓝冰
最大发射波长	710nm
最大激发波长	684nm

生物相关数据

质量控制

请选择批次查看:

纯度 ≥ 98.00%

COA (Certificate Of Analysis)MS (Mass Spectrometry)NMR (Nuclear Magnetic Resonance)MSDS (Material Safety Data Sheet)

操作说明

Protocol Datasheet

APExBIO 顾客使用本产品发表的 40 篇科研文献

1. Xueting Pan, Ying Zhu, et al. "MOF-based Nanoparticles Enabling Blood-Brain Barrier Crossing for Non-Invasive Selective Sononeuromodulation in Epilepsy." Biomaterials. 2026 May 25:335:124339. PMID: 42229302

2. Liwei Yan, Runze Yang, et al. "Bioadhesive Hydrogel With Polyphenol‐Armored Nanogene Rejuvenates Chondrocyte Senescence for Aged Osteoarthritis Therapy." Adv Sci (Weinh). 2026 May 20:e00014. PMID: 42160085

3. Jingbo Zhang, Yajing Bai, et al. "Structural characterization, functional properties, bioactivity and oral absorption characterstics of Oudemansiella raphanipies polysaccharides." Food Chem X. 2025 Sep 24;31:103076. PMID: 41080129

4. Yu Wang, Hongrui Fan, et al. "Mitocytosis-Inducing Nanoparticles Alleviate Gemcitabine Resistance via Dual Disruption of Pyrimidine Synthesis and Redox Homeostasis in Pancreatic Ductal adenocarcinoma." Biomaterials. 2025 Aug 13:325:123630. PMID: 40818322

5. Zhiqiang Chen, Bo Wang, Jianwei Zheng et al. "Reprogramming tumor-associated macrophages and blocking PD-L1 via engineered outer membrane vesicles to enhance T cell infiltration and cytotoxic functions." J Nanobiotechnol 23, 514 (2025) PMID: 40660251

6. Siyuan Peng, Wentao Wu, et al. "Nanoigniter-Integrated Microneedle Patches for Boosted Photothermal-Mediated Multimodal Therapy: In Situ Tumor Microenvironment Igniting Strategy." ACS Nano. 2025 Jul 1;19(25):22931-22952. PMID: 40513082

7. Zeyu Wang, Xue Wang, Hongsheng Fang, et al. "2D Indium‐Vacancy‐Rich ZnIn2S4 Nanocatalysts for Sonocatalytic Cancer Suppression by Boosting Cancer‐Cell Pyroptosis." Adv Mater. 2025 Jun;37(24):e2414432. PMID: 40207706

8. Zheyu Kang, Linfu Chen, et al. "A polyvalent vaccine for selectively killing tumor-associated bacteria to prevent cancer metastasis." Sci Adv.2025 Mar 14;11(11):eadt0341. PMID: 40085697

9. Xu Gong, Shan Liu, et al. "Oral delivery of therapeutic proteins by engineered bacterial type zero secretion system." Nat Commun. 2025 Feb 21;16(1):1862. PMID: 39984501

10. Ruihong Yu, Lulu Zhang, Zuchen Song. "Polyethyleneimine-modified Laminarin nanoparticles enhance antigen delivery to lymph nodes by inducing transient inflammatory responses at the injection site." Materials Today Bio Volume 35, December 2025, 102462

11. Jian Li, Xueting Pan, He Li. "Ultrasound‐Triggered Biomimetic Piezo‐Nanoplatforms for Non‐Invasive Epilepsy Treatment." Advanced Functional Materials 03 November 2025

12. Lizhuo Zhang, Qingqing Feng, et al. "Antigen-Targeting Inserted Nanomicelles Guide Pre-Existing Immunity to Kill Head and Neck Cancer." ADVANCED SCIENCE Volume12, Issue18 May 15, 2025 2410629

13. Zhenyuan Wei, Xiaoxiao Li, et al. "Programmable DNA‐Peptide Conjugated Hydrogel via Click Chemistry for Sequential Modulation of Peripheral Nerve Regeneration." ADVANCED SCIENCE Volume35, Issue23 June 5, 2025 2419915

14. Wei Shu, Yue Jiang, et al. "Developing intranasally administered electro - responsive nanodrugs for rapid epilepsy treatment." Volume 64, October 2025, 102808

15. Weiqi Zhang, Yinghua Zeng, et al. "An in-situ peptide-antibody self-assembly to block CD47 and CD24 signaling enhances macrophage-mediated phagocytosis and anti-tumor immune responses." Nat Commun. 2024 Jul 6;15(1):5670. PMID: 38971872

16. Wentao Jiang, Huizi Xu, et al. "Artificial Neutrophils Against Vascular Graft Infection." Adv Sci (Weinh). 2024 Jun 14:e2402768. PMID: 38874399

17. Xuemei Lu, et al. "Engineered exosomes loaded with M1–8 peptide for targeted therapy of hepatocellular carcinoma." APPLIED materialstoday. Volume 37, April 2024, 102071

18. Ziliang Dong, Lei Zhang, et al. "Procoagulant CaCO3-embedded embolic microspheres can potentiate transcatheter arterial embolization of hepatocellular carcinoma." Nano Today. Volume 55, April 2024, 102216

19. Zhengchao Zhang, Dejun Ding, et al. "Supramolecular Nanozyme System Based on Polydopamine and Polyoxometalate for Photothermal-Enhanced Multienzyme Cascade Catalytic Tumor Therapy." ACS Appl Mater Interfaces. 2023 Aug 16;15(32):38214-38229. PMID: 37535452

20. Dan Yang, Jing Ning, et al. "Local Sustained Chemotherapy of Pancreatic Cancer Using Endoscopic Ultrasound-Guided Injection of Biodegradable Thermo-Sensitive Hydrogel." Int J Nanomedicine. 2023 Jul 20;18:3989-4005. PMID: 37496690

21. Debin Zheng, Jingfei Liu, et al. "Enzyme-instructed and mitochondria-targeting peptide self-assembly to efficiently induce immunogenic cell death." Acta Pharm Sin B. 2022 Jun;12(6):2740-2750. PMID: 35755291

22. Shaodan Ma, Shunan Gu, et al. "Robust drug bioavailability and safety for rheumatoid arthritis therapy using D-amino acids-based supramolecular hydrogels." Mater Today Bio. 2022 May 19;15:100296. PMID: 35665233

23. Qi Wang , Ting Li, et al. "Engineered Exosomes with Independent Module/Cascading Function for Therapy of Parkinson's Disease by Multistep Targeting and Multistage Intervention Method." Adv Mater. 2022 Apr 18;e2201406. PMID: 35435282

24. Zi-Yan Wu, Jian-Min Shen, et al. "pH/Enzyme dual sensitive and nucleus-targeting dendrimer nanoparticles to enhance the antitumour activity of doxorubicin." Pharm Dev Technol. 2022 May 21;1-15. PMID: 35350969

25. Yuanyuan Li, Yangye Jin, et al. "Cyclo (RGD) peptide-decorated silver nanoparticles with anti-platelet potential for active platelet-rich thrombus targeting." Nanomedicine. 2022 Jan 14;41:102520. PMID: 35038589

26. Ziyu Wu, Rui Wu, et al. "Multi‐Pathway Microenvironment Regulation for Atherosclerosis Therapy Based on Beta‐Cyclodextrin/L‐Arginine/Au Nanomotors with Dual‐Mode Propulsion." Small. 2021 Dec 16;e2104120. PMID: 34918450

27. Xiaoyun Li, Rui Wu, et al. "Near-Infrared Light-Driven Multifunctional Tubular Micromotors for Treatment of Atherosclerosis." ACS Appl Mater Interfaces. 2021 Jul 7;13(26):30930-30940. PMID: 34156244

28. Mirza, Amar Naeem. "The Regulation of GLI1 in Basal Cell Carcinoma." Stanford University. 2021

29. Keman Cheng, Tao Du, et al. "Dual-Antigen-Loaded Hepatitis B Virus Core Antigen Virus-like Particles Stimulate Efficient Immunotherapy Against Melanoma." ACS Appl Mater Interfaces. 2020 Nov 18. PMID: 33205941

30. Qinjun Chen, Yongqing He, et al. "Penetrable Nanoplatform for." Cold" Tumor Immune Microenvironment Reeducation." Adv Sci (Weinh). 2020 Jul 29;7(17):2000411. PMID: 32995118

31. Qian Xu, Zheng Zhang, et al. "Tropism-facilitated delivery of CRISPR/Cas9 system with chimeric antigen receptor-extracellular vesicles against B-cell malignancies." J Control Release. 2020 Oct 10;326:455-467. PMID: 32711027

32. Wang L, Yang C, et al. "Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma." Theranostics. 2020;10(15):6674-6694. PMID: 32550897

33. Jiang C, Qi Z, et al. "Dynamically enhancing plaque targeting via a positive feedback loop using multifunctional biomimetic nanoparticles for plaque regression." J Control Release. 2019 Jul 8. pii: S0168-3659(19)30394-3. PMID: 31295543

34. Zou S, Duan B, et al. "Inhibition of tumor growth by β-glucans through promoting CD4(+) T cell immunomodulation and neutrophil-killing in mice." Carbohydr Polym. 2019 Jun 1;213:370-381. PMID: 30879681

35. Cheng M, Zhang Y, et al. "One-pot synthesis of acid-induced in situ aggregating theranostic gold nanoparticles with enhanced retention in tumor cells." Biomater Sci. 2019 Mar 6. PMID: 30839987

36. Jianmin Shen, Jing Chen, et al. "Enhanced lysosome escape mediated by 1, 2-dicarboxylic-cyclohexene anhydride-modified poly-L-lysine dendrimer as a gene delivery system." Journal Pre-proof. 10 December 2019. pii: S1818-0876(19)30883-9

37. Xiaolei Zhang, Chuangnian Zhang, et al. "Dual pH-responsive." charge-reversal like" gold nanoparticles to enhance tumor retention for chemo-radiotherapy." Nano Research. 27 September 2019

38. Sheridan J.S. Carrington. "Differential Glycosylation of the Inwardly Rectifying Potassium Channel Kir7.1 by G protein-coupled Receptors." Vanderbilt University. 2019

39. Jiang C, Qi Z, et al. "ATP-responsive low-molecular-weight polyethylenimine-based supramolecular assembly via host-guest interaction for gene delivery." Biomacromolecules. 2018 Dec 5. PMID: 30516950

40. Lu J, Zhao Y, et al. "Biofunctional polymer-lipid hybrid high density lipoprotein-mimicking nanoparticles loading anti-miR155 for combined antiatherogenic effects on macrophages." Biomacromolecules. 2017 Aug 3. PMID: 28738148

摩尔浓度计算器

质量

= 浓度

x 体积

x 分子量

*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。