Cy5 NHS ester
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 2mg | ￥1000.00 ￥700.00 | 现货 | |
| 5mg | ￥1320.00 ￥924.00 | 现货 | |
| 10mg | ￥1936.00 ￥1355.20 | 现货 | |
| 25mg | ￥3740.00 ￥2618.00 | 现货 | |
| 50mg | ￥4845.00 ￥3391.50 | 现货 | |
| 100mg | ￥7999.00 ￥5599.30 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:
[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
产品性质
| CAS号 | 146368-14-1 |
| 分子式 | C37H43N3O10S2 |
| 分子量 | 753.88 |
| 小分子别名 | CY5-SE; Sulfo-Cyanine5 Succinimidyl Ester |
| 化学名称 | 2-((1E,3E)-5-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| 溶解度 | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
| SMILES | CC[N+]1=C(/C=C/C=C/C=C2N(CCCCCC(ON3C(CCC3=O)=O)=O)C(C=CC(S(O)(=O)=O)=C4)=C4C/2(C)C)C(C)(C)C5=C1C=CC(S([O-])(=O)=O)=C5 |
| 存储条件 | -20°C干燥避光 |
| 运输条件 | 蓝冰 |
| 最大发射波长 | 662nm |
| 最大激发波长 | 646nm |
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