Cy5 NHS ester
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:
[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
- 1. Guo M, Du W, et al. "Ultra-Early Diagnosis of Acute Myocardial Infarction in Rats Using Ultrasound Imaging of Hollow Double-Layer Silica Nanospheres." Adv Healthc Mater. 2020;9(3):e1901155. PMID:31867893
- 2. Hu Y, Hou Y, et al. "Polysarcosine as an Alternative to PEG for Therapeutic Protein Conjugation." Bioconjug Chem. 2018 Jul 18;29(7):2232-2238. PMID:29863329
- 3. Batzaya Nyambat, Chih-Hwa Chen, et al."Genipin-Crosslinked Adipose Stem Cell Derived Extracellular Matrix-Nano Graphene Oxide Composite Sponge for Skin Tissue Engineering." Journal of Materials Chemistry B. 2018 January 15.
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
M.Wt | 761.84 |
Formula | C36H40NaN3O10S2 |
Solubility | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
SDF | Download SDF |
Canonical SMILES | CC(C1=CC([Na])=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=CC=C(S(=O)([O-])=O)C=C5C3(C)C.O=S(=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 646 | 最大发射光波 (nm) | 662 |
消光系数 (M-1cm-1) | 271000 | 量子产量 | 0.28 |
CF260 | 0.04 | CF280 | 0.04 |
质量控制和MSDS
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