HPF
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HPF是一种细胞可渗透的，光稳定的羟苯基荧光素，可作为检测高活性氧（hROS）的高度灵敏的荧光探针[1]。有许多荧光试剂，例如2,7-二氯二氢荧光素（DCDHF），可用于检测自由基，但是它们容易被光氧化以及许多非自由基氧化剂（例如过氧化氢）氧化，因此存在很大的局限性。而HPF仅被高活性氧（hROS）氧化，例如氢氧自由基，过氧亚硝酸盐和过氧化物酶/ H2O2系统产生的hROS。该化合物对其他活性氧（ROS），例如次氯酸盐，单线态氧，超氧化物，过氧化氢，一氧化氮和烷基过氧化物几乎没有活性。
HPF本身没有荧光，当与hROS（如羟基自由基和过亚硝酸盐）反应被氧化后，HPF转换为高荧光分子荧光素，激发/发射最大值分别为490/515 nm。
由于HPF不会与一氧化氮，超氧化物和过氧化氢反应，因此可以与一氧化氮和超氧化物区分开来检测亚硝酸盐。由于HPF不与次氯酸盐（-OCl）反应，因此可以与检测-OCl的APF（氨基苯基荧光素）结合使用[2,3]。
References:
[1] Kobayashi H, Ogawa M, Alford R, et al. New strategies for fluorescent probe design in medical diagnostic imaging. Chem Rev. 2010;110(5):2620–2640.
[2] Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem. 2003 Jan 31;278(5):3170-5.
[3] Choung YH, Taura A, Pak K, et al. Generation of highly-reactive oxygen species is closely related to hair cell damage in rat organ of Corti treated with gentamicin. Neuroscience. 2009;161(1):214–226.
- 1. Yumeng Lian, Yi Wang, et al. "Peroxidase-like and oxidase-like nanozyme activities of reusable Mn-Co-S-Se/Ni foam for antibacterial application." Colloids and Surfaces A: Physicochemical and Engineering Aspects. Volume 626, 5 October 2021, 127010.
- 2. CHEN Chao, WANG Yi, et al. "Bifunctional nanozyme activities of layered double hydroxide derived Co-Al-Ce mixed metal oxides for antibacterial application." Journal of Oceanology and Limnology. Mar. 21, 2020.
- 3. Wang J, Wang Y, et al. "Exploring the bactericidal performance and application of novel mimic enzyme Co4S3." J Colloid Interface Sci. 2020;561:327–337. PMID: 31771873
- 4. YiWangacd, ChaoChenabcd, et al. "Bifunctionalized novel Co-V MMO nanowires: Intrinsic oxidase and peroxidase like catalytic activities for antibacterial application." Applied Catalysis B: Environmental Available online 5 October 2019, 118256. PMID: 31666990
| Physical Appearance | A solution in acetate. To change the solvent, simply evaporate the acetate containing under a gentle stream of nitrogen and immediately add the solvent of choice. |
| Storage | Store at -20°C |
| M.Wt | 424.4 |
| Cas No. | 359010-69-8 |
| Formula | C26H16O6 |
| Solubility | ≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide |
| Chemical Name | 3'-hydroxy-6'-(4-hydroxyphenoxy)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| SDF | Download SDF |
| Canonical SMILES | OC(C=C1)=CC=C1OC2=CC3=C(C=C2)C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C=C6O3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
