SB590885
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB590885是有效的B-Raf激酶选择性抑制剂,其Ki值为0.16 nM[1].
SB590885是有效的致癌性B-Raf蛋白激酶抑制剂,其Ki值为0.16 nM.相对于C-Raf,SB590885更有效地抑制B-Raf.SB590885对C-Raf的Ki值为1.72 nM.SB590885具有高度选择性,对48种其它人类激酶无效,如Abl\AMPK\CK1\CK2以及ERK2.据发现,SB590885与B-Raf的ATP结合口袋结合,稳定B-Raf的活性构象.在表达B-Raf V600E致癌突变的肿瘤细胞中,SB590885减少ERK磷酸化并显示出抗增殖活性.除正常黑色素细胞和表达野生型B-Raf的原代黑色素瘤细胞外,正常细胞和不表达突变B-Raf的肿瘤细胞均对SB590885不敏感.此外,在表达突变B-Raf的恶性细胞中,SB590885还降低了其转化与致瘤性[1].
参考文献：
[1] King A J, Patrick D R, Batorsky R S, et al. Demonstration of a genetic therapeutic index for tumors expressing oncogenic BRAF by the kinase inhibitor SB-590885. Cancer research, 2006, 66(23): 11100-11105.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 453.55 |
| Cas No. | 405554-55-4 |
| Formula | C27H27N5O2 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥4.54 mg/mL in DMSO with gentle warming |
| Chemical Name | N,N-dimethyl-2-[4-[(4Z)-4-(1-nitroso-2,3-dihydroinden-5-ylidene)-5-(1H-pyridin-4-ylidene)-1H-imidazol-2-yl]phenoxy]ethanamine |
| SDF | Download SDF |
| Canonical SMILES | CN(C)CCOC1=CC=C(C=C1)C2=NC(=C3C=CC4=C(CCC4=C3)N=O)C(=C5C=CNC=C5)N2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | SB590885是有效的B-Raf抑制剂,其Ki值为0.16 nM. | |||||
| 靶点 | B-Raf | |||||
| IC50 | 0.16 nM | |||||
质量控制和MSDS
- 批次:
化学结构
