RS-1
RAD51激活剂
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 10mg | ￥690.00 | 现货 | |
| 25mg | ￥1309.00 | 现货 | |
| 50mg | ￥1990.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
RS-1 is a stimulatory compound of RAD51, a key player in the HR complex. RS-1 could increase the DNA binding activity of RAD51 and function in vivo to enhance the homologous recombination activity of hRAD51 by promoting the formation of active presynaptic filaments [1].
The hRAD51 protein could bind with single- and double-stranded DNA and exhibit DNA-dependent ATPase activity. Rad51 mediates homology recognition and initiates strand exchange [2]. Chromosome analysis revealed that Rad51‐deficient vertebrate cells accumulated chromosomal breaks in the G2/M phase of the cell cycle prior to cell death. Rad51 played an essential role in the repair of spontaneously occurring chromosome breaks in proliferating cells of higher eukaryotes [3].
RS-1 treatment promoted significant antitumor responses in a mouse mode. It can be used to kill human cancer cells, and that its toxicity is modulated by both RAD51 and RAD54 translocase expression levels [4]. RS-1 effectively improved the knock-in rates in the TALEN- and the Cas9-mediated genome-editing systems in rabbits. RS-1 showed little toxic effects on the overall animal health and reproduction. RS-1 enhanced Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo. RS-1 treatment (15 μM) appeared to enhance the blastocyst development in embryos. Treating embryos with RS-1 at 7.5 μM resulted in 26.1% knock-in rate [5].
References:
[1]. Jayathilaka K, Sheridan S D, Bold T D, et al. A chemical compound that stimulates the human homologous recombination protein RAD51[J]. Proceedings of the National Academy of Sciences, 2008, 105(41): 15848-15853.
[2]. Baumann P, Benson F E, West S C. Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro[J]. Cell, 1996, 87(4): 757-766.
[3]. Sonoda E, Sasaki M S, Buerstedde J M, et al. Rad51‐deficient vertebrate cells accumulate chromosomal breaks prior to cell death[J]. The EMBO journal, 1998, 17(2): 598-608.
[4]. Mason J M, Logan H L, Budke B, et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors[J]. Cancer research, 2014, 74(13): 3546-3555.
[5]. Song J, Yang D, Xu J, et al. RS-1 enhances CRISPR/Cas9-and TALEN-mediated knock-in efficiency[J]. Nature communications, 2016, 7.
产品性质
| 物理外观 | Solid |
| CAS号 | 312756-74-4 |
| 分子式 | C20H16Br2N2O3S |
| 分子量 | 524.2 |
| 化学名称 | 4-bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide |
| 溶解度 | insoluble in EtOH; insoluble in H2O; ≥20 mg/mL in DMSO |
| SMILES | BrC1=CC=C(NC(C2=CC=C(Br)C(S(NCC3=CC=CC=C3)(=O)=O)=C2)=O)C=C1 |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | RS-1 是一种 RAD51 激活剂；也能提高 CRISPR/Cas9 介导的基因敲入效率。 |
质量控制
APExBIO 顾客使用本产品发表的 3 篇科研文献
2. Weina Zhang, Mingzhu Wang, et al. "Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer." Nat Commun. 2023 Apr 3;14(1):1838. PMID: 37012254
3. Weina Zhang, Yu Chen, et al. "A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing." Elife. 2020 Jul 9;9:e56008. PMID: 32644042
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