Cytochalasin B
细胞渗透性的肌动蛋白聚合抑制剂
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 1mg | ￥1242.00 | 现货 | |
| 5mg | ￥3428.00 | 现货 | |
| 10mg | ￥5670.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Cytochalasin B is a cell-permeable mycotoxin which binds to the barbed end of actin, reversibly inhibiting the elongation and shortening of actin filaments, with Kd value of 1.4-2.2 nM for F-actin[1].
Cytochalasin B (0.1-10 μM) shows inhibitory effect on multiple murine cancer cell lines[2]. In addition, Cytochalasin B accelerates the transformation from F-actin to G-actin, lowering the content of F-actin and significantly increasing G-actin bands during postmortem conditioning[3].
Cytochalasin B dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50 mg/kg produces 10 % long-term survival in the multidrug resistant P388/ADR cohort, and 40 % long-term survival in the drug sensitive P388/S cohort[2].
References:
[1]. Theodoropoulos P A, Gravanis A, Tsapara A, et al. Cytochalasin B may shorten actin filaments by a mechanism independent of barbed end capping. Biochemical Pharmacology, 1994, 47(10): 1875-1881.
[2]. Trendowski M, et al. Chemotherapy with cytochalasin congeners in vitro and in vivo against murine models. Invest New Drugs. 2015 Apr;33(2):290-9.
[3]. Zhou C, et al. The effect of Cytochalasin B and Jasplakinolide on depolymerization of actin filaments in goose muscles during postmortem conditioning. Food Res Int. 2016 Dec;90:1-7.
产品性质
| 物理外观 | A crystalline solid |
| CAS号 | 14930-96-2 |
| 分子式 | C29H37NO5 |
| 分子量 | 479.61 |
| 化学名称 | (3S,3aS,4S,6S,6aS,7E,10R,14R,15E,18aS)-3-benzyl-6,14-dihydroxy-4,10-dimethyl-5-methylene-3,3a,4,5,6,6a,9,10,11,12,13,14-dodecahydro-1H-[1]oxacyclotetradecino[2,3-d]isoindole-1,17(2H)-dione |
| 溶解度 | ≤20mg/ml in ethanol;20mg/ml in DMSO;30mg/ml in dimethyl formamide |
| SMILES | O=C1/C=C/[C@@H](CCC[C@H](C/C=C/[C@]([H])([C@@]23O1)[C@@H](C([C@H]([C@]2([C@@H](NC3=O)CC4=CC=CC=C4)[H])C)=C)O)C)O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
质量控制
操作说明
APExBIO 顾客使用本产品发表的 1 篇科研文献
- 1. Wei P, Ning M, et al. "Spiroplasma eriocheiris entered Drosophila Schneider 2 cells and relied on clathrin-mediated endocytosis and macropinocytosis." Infect Immun. 2019 Aug 26. pii: IAI.00233-19. PMID:31451616
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