PCI-32765 Racemate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PCI-32765是一种Bruton酪氨酸激酶抑制剂(BTK),IC50值为0.5 nM[1] .
PCI-32765是共价结合和不可逆的BTK抑制剂,结合到 ATP结合域的Cys-481上.在细胞实验中,PCI-32765抑制B细胞BTK的磷酸化作用(IC50值为11 nM),以及下游底物磷酸肌醇磷脂酶Cγ(PLC γ)和ERK的磷酸化作用.PCI-32765对B细胞淋巴瘤具有体内活性.在CLL细胞中,PCI-32765通过抑制BCR依赖的UDP葡萄糖神经酰胺糖基转移酶的表达从而诱导细胞凋亡[1].
PCI-32765在体内口服有效.它诱导CLL患者第一周治疗期间淋巴细胞增多.PCI-32765对自身免疫性疾病也有效.在MRL-Fas狼疮模型中,PCI-32765抑制胶原诱导的关节炎以及自身抗体的产生和肾脏疾病的发展.PCI-32765也减少FCγRIII诱导的促炎性细胞因子生成[1].
参考文献：
[1] Burger JA, Buggy JJ. Bruton tyrosine kinase inhibitor ibrutinib (PCI-32765). Leuk Lymphoma. 2013 Nov;54(11):2385-91.
| Storage | Store at RT |
| M.Wt | 440.5 |
| Cas No. | 936563-87-0 |
| Formula | C25H24N6O2 |
| Solubility | Soluble in DMSO |
| Chemical Name | 1-[3-[4-amino-3-(4-phenoxyphenyl)pyrazolo[3,4-d]pyrimidin-1-yl]piperidin-1-yl]prop-2-en-1-one |
| SDF | Download SDF |
| Canonical SMILES | C=CC(=O)N1CCCC(C1)N2C3=C(C(=N2)C4=CC=C(C=C4)OC5=CC=CC=C5)C(=NC=N3)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
