ME0328
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ME0328是PARP的强效选择性抑制剂，对PARP3和PARP1的IC50分别为0.89 μM和 6.3 μM [1]。
聚合酶（PARP）是一个蛋白质家族，主要参与程序性细胞死亡和DNA修复。PARP的过度激活可降低细胞NAD+的存储，诱导ATP消耗和细胞死亡。
ME0328是PARP的强效选择性抑制剂。ME0328选择性抑制ADP-核糖基转移-3-聚合酶-3（ARTD3），IC50为0.89 μM，抑制ARTD1和ARTD2的IC50分别为6.3和10.8 μM。在人肺泡基底上皮细胞（A549）中，使用10 μM的 ME0355处理，接着进行γ射线照射，以浓度依赖性方式显著延迟γH2AX-foci的分辨率，这表明，ME0328抑制了细胞中的ARTD3。在未受辐射的人A549和小鼠MRC5细胞中，10 μM ME0328未表现出细胞毒性[1]。
参考文献：
[1]. Lindgren AE, Karlberg T, Thorsell AG, et al. PARP inhibitor with selectivity toward ADP-ribosyltransferase ARTD3/PARP3. ACS Chem Biol, 2013, 8(8): 1698-1703.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 321.37 |
| Cas No. | 1445251-22-8 |
| Formula | C19H19N3O2 |
| Solubility | ≥71.43 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 3-(4-oxo-1H-quinazolin-2-yl)-N-[(1S)-1-phenylethyl]propanamide |
| SDF | Download SDF |
| Canonical SMILES | CC(C1=CC=CC=C1)NC(=O)CCC2=NC(=O)C3=CC=CC=C3N2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| ME0328 is a potent and selective PARP inhibitor with IC50 of 0.89 μM for PARP3, about 7-fold selectivity over PARP1. | ||||||
| Targets | PARP3 | PARP1 | ||||
| IC50 | 0.89μM | 6.3μM | ||||
化学结构
