α-Amanitin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Jiangli Lang, Chen Yang, et al. "High Glucose Activates ERK1/2 to Stabilize AP1 and Increase MMP9 Expression in Diabetic Foot Ulcers." Exp Cell Res. 2021 Mar 3;112550. PMID:33675806
- 2. Huo X, Ji L, et al. "The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation." Mol Cell. 2019 Oct 28. pii: S1097-2765(19)30761-0. PMID:31677973
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 918.97 |
| Cas No. | 23109-05-9 |
| Formula | C39H54N10O14S |
| Solubility | ≥1 mg/mL in H2O; Soluble in Ethanol |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Mouse blastocysts |
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Reaction Conditions |
1.1 μg/ml α-amanitin |
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Applications |
α-Amanitin inhibited the incorporation of 3H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%. |
| Animal experiment:[1] | |
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Animal models |
Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice |
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Dosage form |
0 ~ 1 μg/ml α-amanitin 48 h |
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Applications |
Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680. |
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化学结构
相关生物数据
相关生物数据
相关生物数据
