Insulin (human) recombinant expressed in yeast
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Insulin-MTX对胰岛素受体的IC50值为5.01 nmol/L。
胰岛素属于肽类激素,由胰腺β细胞产生。胰岛素促进葡萄糖吸收进入肌肉与脂肪组织中,在维持全身葡萄糖稳态中起着重要作用。胰腺β细胞的自身免疫性损伤导致胰岛素合成补足,促进I型和II型糖尿病发展。
体外:Methotrexate(MTX)共价结合胰岛素。Insulin-MTX复合物与125I-Insulin竞争胰岛素受体,其作用与胰岛素相当。Insulin-MTX复合物的IC50值与Ki值分别93.82 nM和91.88 nM,而胰岛素的IC50值与Ki值分别为5.01 nM和4.85 nM[1]。
体内:以往研究表明,在SJL小鼠中,胰岛素降低伸肌的葡萄糖摄取量,但葡萄糖基础摄取率无改变。在另一项小鼠研究中,敲除3T3-L1脂肪细胞的TC10α(保留TC10β)抑制胰岛素诱导的葡萄糖摄取以及GLUT4易位[2]。
临床试验:胰岛素水平降低可引起糖尿病。因而,胰岛素用于治疗某些糖尿病。通过重组DNA技术生产临床用的生物合成人胰岛素。胰岛素通常采取皮下注射给药途径。此外,吸入式胰岛素已在美国市场上出售(https://en.wikipedia.org/wiki/Insulin#Medication_uses)。
参考文献:
[1] Ou X, Kuang A, Liang Z, Peng X, Zhong Y. The binding characteristics of insulin-MTX to insulin receptor. Hua Xi Yi Ke Da Xue Xue Bao. 2001 Dec;32(4):538-40.
[2] Leney SE, Tavaré JM. The molecular basis of insulin-stimulated glucose uptake: signalling, trafficking and potential drug targets. J Endocrinol. 2009 Oct;203(1):1-18.
Physical Appearance | White solid |
Storage | Store at -20°C |
M.Wt | 5807.57 |
Cas No. | 11061-68-0 |
Formula | C257H383N65O77S6 |
Solubility | ≥58.1 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次: