ID-8
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ID-8是一种DYRK小分子抑制剂[1].
双特异性酪氨酸磷酸化调节激酶(DYRK)是一种催化酪氨酸和丝氨酸/苏氨酸残基磷酸化的酶.
ID-8是一种DYRK小分子抑制剂.在HES2细胞中,ID-8(0.5 μM)可提高1.1%的hESC存活率.ID-8和Wnt3a联合使用可提高1.7%存活率,并能完全抑制Wnt诱导的形态学分化.并且,ID-8可显著降低Wnt诱导的分化标记基因GATA6\GSC\SOX17和CDX2的表达.在hESCs中,ID-8可通过抑制DYRK,提高Wnt介导的hESC增殖和存活.并且,DYRK家族是ID-8的直接靶标[1].在胚胎干细胞(ESC)中,ID-8(10 μM)可在无MEF\血清或LIF的培养物中刺激增殖.ID-8诱导的ESC具有形成所有三种胚层衍生组织的能力,如肝脏\神经元和肌肉.并且,ID-8可提高ESC分化以及Nanog\Sox2和Rex-1水平[2].在无血清培养基中培养的小鼠ESC中,ID-8保留了其自我更新能力和多能性[3].
参考文献：
[1]. Hasegawa K, Yasuda SY, Teo JL, et al. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med, 2012, 1(1): 18-28.
[2]. Miyabayashi T, Yamamoto M, Sato A, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem, 2008, 72(5): 1242-1248.
[3]. Firestone AJ, Chen JK. Controlling destiny through chemistry: small-molecule regulators of cell fate. ACS Chem Biol, 2010, 5(1): 15-34.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 298.29 |
| Cas No. | 147591-46-6 |
| Formula | C16H14N2O4 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥13.95 mg/mL in DMSO |
| Chemical Name | 1-(4-methoxyphenyl)-2-methyl-3-nitroindol-6-ol |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C2=C(N1C3=CC=C(C=C3)OC)C=C(C=C2)O)[N+](=O)[O-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
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细胞系 |
D3ES胚胎干细胞（ESC） |
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溶解方法 |
该化合物在DMSO中的溶解度>13.95mg/mL。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
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反应条件 |
10 μM; 超过1个月 |
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应用 |
在无血清培养基中培养的D3ES胚胎干细胞（ESC）中，ID-8能够使ESC在未分化状态下增殖至少30天。维持在补充有ID-8的培养基中的细胞可以分化成各种细胞类型，例如神经元，肌肉和肝脏。从长期培养物中去除ID-8导致ESC分化。 ID-8通过Sox2-Oct3/4激活，维持Nanog基因表达，可逆维持ESCs的长期培养。 |
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References: [1]. Miyabayashi T, Yamamoto M, Sato A, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem, 2008, 72(5): 1242-1248. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
