HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribe™共转录mRNA合成试剂盒（EZ Cap Reagent AG (3' OMe)、5-moUTP、T7）专为体外快速生产含Cap 1加帽的mRNA而设计。通过使用T7 RNA聚合酶共转录掺入帽类似物（EZ Cap Reagent AG (3' OMe), 货号B8178）使mRNA发生Cap 1加帽，确保mRNA的高翻译效率。添加5-moUTP（货号B8061）可降低宿主细胞免疫反应。另外，poly(A)尾能稳定真核生物mRNA，并提高翻译起始效率。本试剂盒推荐使用3 ’端自带加尾序列的DNA转录模板进行mRNA的poly(A)尾稳定添加（通常为100-120 A）。
使用该试剂盒合成的RNA在生物学实验中有许多应用，例如体外翻译，反义RNA和RNAi实验，RNA疫苗，RNA结构和功能研究，核酶生物化学，RNase蛋白实验和基于探针的杂交印迹。
该试剂盒包含足够的试剂，可进行25次反应，每次20 μL，并在K1068的基础上调整了组分及反应配比。对于标准20 μL反应体系可获得更高的RNA产量。
| Components | 25 rxn |
| T7 RNA Polymerase Mix | 50 μL |
| 10 × Reaction Buffer | 50 μL |
| ATP (100 mM) | 30 μL |
| 5-moUTP (100 mM) | 25 μL |
| CTP (100 mM) | 25 μL |
| GTP (100 mM) | 25 μL |
| EZ Cap Reagent AG (3' OMe) (100 mM) | 20 μL |
| Control Template (0.5 μg/μL) | 5 μL |
| RNase-free H2O | 0.5 mL |
Store the components at -20°C. | |