High Yield T7 Biotin16 RNA Labeling Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
High Yield T7 Biotin16 RNA Labeling Kit是专门用于体外转录中产生随机Biotin修饰的RNA探针的试剂盒。这种探针非常适合原位杂交和Northern Blot杂交实验。标记的原理类似于Biotin RNA标签混合物的基本标记原理；使用优化的反应缓冲液和T7 RNA聚合酶混合物，Biotin-16-UTP可有效地结合到RNA中，代替其天然对应物UTP。35%的Biotin-16-UTP替代通常可在反应和标记效率之间达到最佳平衡。由此产生的Biotin修饰的RNA探针可以通过荧光光谱检测。
| 组分 | 25 rxns |
| T7 RNA Polymerase Mix | 50 μL |
| 10X Reaction Buffer | 50 μL |
| ATP (20 mM) | 50 μL |
| GTP (20 mM) | 50 μL |
| UTP (20 mM) | 37.5 μL |
| CTP (20 mM) | 50 μL |
| Biotin16-UTP (10mM) | 25 μL |
| Control Template(0.5 μg/μL) | 5 μL |
| RNase-free H2O | 1 mL |
试剂盒储存于-20°C。 | |