eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) (387-394) [Multiple species]
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
氨基酸序列:LEU-GLU-ASP-GLY-PRO-LYS-PHE-LEU。
真核翻译延伸因子1α1(EEF1A1)编码延伸因子-1复合物的α(alpha)亚基,它负责将氨酰tRNA转运到核糖体。这种亚型(alpha 1)表达于脑、胎盘、肺、肝、肾和胰腺中,而另一个亚型(alpha 2)在脑、心脏和骨骼肌中表达。在66%的费尔蒂综合征患者中,这种亚型被确定为自身抗原。研究发现,该基因在许多染色体中具有多个拷贝,其中一些代表不同的假基因1。
在真核生物中存在两种形式的EEF1A,即EEF1A1和EEF1A2,这两者在蛋白质合成中发挥相同的作用2。研究表明,EEF1A2特异性的表达在脑、心脏和骨骼肌中,而EEF1A1在各个器官和组织中广泛分布3。
受损染色体缩合产生四倍体细胞,可以通过一种新型的细胞死亡机制清除细胞,这种机制不依赖于胱天蛋白酶(caspase)诱导的细胞凋亡。细胞死亡与真核翻译延伸因子1α1(EEF1A1 / EF-1a)的表达下调以及其mRNA在加工小体(P小体)的积累相关。重要的是,外源EEF1A1表达可以抑制caspase非依赖性的细胞死亡,通过诱导eEF1A1特异性的短发夹RNA的表达,可以观察到类似的细胞死亡现象2。
Figure1. Structure of eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)
Figure2. Formula of eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) (387-394) [Multiple species]
C43H67N9O13
参考文献:
1. "Entrez Gene: EEF1A1 eukaryotic translation elongation factor 1 alpha 1"
2. Kahns S, LundA, Kristensen P, Knudsen CR, ClarkBF, Cavallius J et al. The elongationfactor 1 A-2 isoform from rabbit: cloning of the cDNA and characterization of the protein. Nucleic Acids Res 1998; 26: 1884–1890
3. Lee S, Francoeur AM, Liu S, Wang E. Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family. J Biol Chem 1992; 267: 24064–24068.
4. Y Kobayashi and S Yonehara. Cell Death and Differentiation (2009) 16, 139–150
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 918.04 |
Formula | C43H67N9O13 |
Synonyms | H2N-Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu-OH |
Solubility | ≥91.8 mg/mL in DMSO; ≥10.44 mg/mL in EtOH; ≥48.6 mg/mL in H2O |
SDF | Download SDF |
Canonical SMILES | NC(CC(C)C)C(NC(CCC(O)=O)C(NC(CC(O)=O)C(NCC(N(CCC1)C1C(NC(CCCCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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