Dpn I
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Dpn Ⅰ是一种Dam甲基化敏感的限制性内切酶,识别GA/TC序列,只有当序列中的腺嘌呤N6-甲基化后才能对DNA进行切割。哺乳动物DNA的CpG甲基化与Dpn Ⅰ酶切位点重叠时会阻断其酶切,另外Dpn Ⅰ对Dcm甲基化无酶切作用。该产品适用于质粒DNA、PCR产物或基因组DNA等的快速酶切反应,被广泛应用于基因分型、分子克隆、Southern印迹、SNP分子标记、RFLP技术等众多研究领域。
其识别序列和切割位点如下:
5'…… G Am6 ↓T C…… 3'
3'…… C T↑ Am6G…… 5'
Components | 500 U | 1000 U | 5000 U |
Dpn I (20 U/μL) | 0.025 mL | 0.05 mL | 0.25 mL |
10X Dpn I Reaction Buffer | 0.5 mL | 1 mL | 5 mL |
Store the components at -20 °C. |