Dpn I
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Dpn Ⅰ是一种Dam甲基化敏感的限制性内切酶，识别GA/TC序列，只有当序列中的腺嘌呤N6-甲基化后才能对DNA进行切割。哺乳动物DNA的CpG甲基化与Dpn Ⅰ酶切位点重叠时会阻断其酶切，另外Dpn Ⅰ对Dcm甲基化无酶切作用。该产品适用于质粒DNA、PCR产物或基因组DNA等的快速酶切反应，被广泛应用于基因分型、分子克隆、Southern印迹、SNP分子标记、RFLP技术等众多研究领域。
其识别序列和切割位点如下：
5'…… G Am6 ↓T C…… 3'
3'…… C T↑ Am6G…… 5'
| Components | 500 U | 1000 U | 5000 U |
| Dpn I (20 U/μL) | 0.025 mL | 0.05 mL | 0.25 mL |
| 10X Dpn I Reaction Buffer | 0.5 mL | 1 mL | 5 mL |
Store the components at -20 °C. | |||