RNase H (RNase-free)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
RNase H，即核糖核酸酶H，是一种核糖核酸内切酶，可特异性地水解DNA-RNA结合链中RNA磷酸二酯键。RNase H具有双二价金属离子催化机制，如Mg2+和Mn2+直接参与催化功能。RNase H不能水解单链或双链DNA或RNA中的磷酸二酯键。本试剂盒可用于cDNA第二链合成前去除DNA与mRNA复合结构中的mRNA、通过互补的DNA序列实现对RNA的定点剪切、DNA-RNA杂交体的鉴定、除去杂交到poly(dT)上的mRNA中的poly(A)、体外多腺苷酸化反应(polyadenylation reaction)产物研究等方面。
| Components | 500 U | 1000 U | 5000 U |
| RNase H (RNase-free) | 0.1 ml | 0.2 ml | 1 ml |
| 10X RNase H Reaction Buffer | 0.5 ml | 1 ml | 1.5ml x2 |
Store at -20 °C. | |||