T4 Endonuclease Ⅶ
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T4 Endonuclease Ⅶ是T4噬菌体基因49的产物,分子量约为18 kDa。T4核酸内切酶VII在体内参与DNA包装、基因重组和错配修复。在体外也被证明可以降解错配的单碱基、异源双链loop环和分支DNA,如four-way Holliday结构和three-way Y结构。该产品可用于CRISPR/Cas9、TALEN和其它基因编辑工具形成的突变体检测,以及基因点突变和SNP的检测等。
Components | 500 U | 1000 U | 2500 U |
T4 Endonuclease Ⅶ (10 U/μL) | 0.05 mL | 0.1 mL | 0.25 mL |
10X T4 Endonuclease Ⅶ Reaction Buffer | 0.5 mL | 1 mL | 2.5 mL |
Store the components at -20 °C. |