DMAT
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Lee S, Jeon YM, et al. "PTK2/FAK regulates UPS impairment via QSTM1/p62 phosphorylation in TARDBP/TDP-43 proteinopathies." Autophagy. 2019 Nov 5:1-17. PMID:31690171
- 2. Zhou Y, Su JM, et al. "Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles." J Virol. 2019 Aug 2. pii: JVI.00948-19. PMID:31375591
- 3. Zhao Z, Wang L, et al."Regulation of MLL/COMPASS stability through its proteolytic cleavage by taspase1 as a possible approach for clinical therapy of leukemia." Genes Dev. 2019 Jan 1;33(1-2):61-74. PMID:30573454
- 4. Shinrye Lee, Yu-Mi Jeon, et al. "PTK2 regulates the UPS impairment via p62 phosphorylation in TDP-43 proteinopathy." bioRxiv.2018.June 25.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 476.79 |
Cas No. | 749234-11-5 |
Formula | C9H7Br4N3 |
Synonyms | Casein kinase II Inhibitor;CK2 Inhibitor |
Solubility | ≥23.85 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
Chemical Name | 4,5,6,7-tetrabromo-N,N-dimethyl-1H-benzimidazol-2-amine |
SDF | Download SDF |
Canonical SMILES | CN(C)C1=NC2=C(N1)C(=C(C(=C2Br)Br)Br)Br |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
人源多能肾上腺皮质细胞系H295R (CRL-2128) |
溶解方法 |
在DMSO中的溶解度>23.9mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月 |
反应时间 |
DMAT溶解于96%乙醇和无类血清的培养基中,终浓度为10-4–10-10 M,72 h |
应用 |
DMAT的使用减少了醛固酮,脱氢表雄酮硫酸盐,雄烯二酮的分泌,导致17-羟孕酮的积累。细胞生长被抑制,并且细胞周期分析显示了轻微的细胞凋亡的诱导。 |
动物实验[2]: | |
动物模型 |
6-8周携带人源肝癌细胞HepG2异种移植物的雄性NMRI小鼠 |
剂量 |
500 μg/kg 溶解于DMSO/PBS混合溶液,每日使用,10天,腹腔注射 |
应用 |
在带有异种移植瘤的模型体内,DMAT的使用通过干扰肿瘤细胞增殖来降低肿瘤生长。DMAT通过干扰NFκB和Wnt信号通路来减少HCC(肝细胞癌)生长。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Lawnicka H1, Kowalewicz-Kulbat M, et al, Anti-neoplastic effect of protein kinase CK2 inhibitor, 2-dimethylamino-4,5,6,7-tetrabromobenzimidazole (DMAT), on growth and hormonal activity of human adrenocortical carcinoma cell line (H295R) in vitro. Cell Tissue Res. 2010 May;340(2):371-9. doi: 10.1007/s00441-010-0960-1. Epub 2010 Apr 6. [2]. Sass G1, Klinger N, et al, Inhibition of experimental HCC growth in mice by use of the kinase inhibitor DMAT. Int J Oncol. 2011 Aug;39(2):433-42. doi: 10.3892/ijo.2011.1037. Epub 2011 May 10. |
质量控制和MSDS
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