DL-threo-PPMP (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DL-threo-PPMP (hydrochloride) is an inhibitor of glucosylceramide synthase [1].
Glucosylceramide synthase (GCS) is a pervasive enzyme inherent to intracellular ceramide metabolism. The enzyme has been involved in catalyzing the transfer of glucose to ceramide, which is the principle committed step in glycolipid biosynthesis. GCS has been involved in the cytotoxic response of cancer cells to chemotherapy. Glucosylceramide synthase-based sphingolipids have been identified as important mediators of a variety of cellular functions, including proliferation, differentiation, development, and cell-cell recognition [2].
DL-threo-PPMP was a ceramide analog. DL-threo-PPMP inhibited the activity of glucosylceramide synthase with an IC50 value between 2 and 20 μM. It has been used to study the role of glucosylceramide synthase in cell growth, apoptosis, and autophagy [1].
References:
[1] Lee L, Abe A, Shayman J A. Improved inhibitors of glucosylceramide synthase[J]. Journal of Biological Chemistry, 1999, 274(21): 14662-14669.
[2] Bleicher R J, Cabot M C. Glucosylceramide synthase and apoptosis[J]. Biochimica et biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2002, 1585(2): 172-178.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 511.2 |
| Cas No. | 139974-41-7 |
| Formula | C29H50N2O3·HCl |
| Synonyms | DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol |
| Solubility | ≤10mg/ml in ethanol;20mg/ml in DMSO;5mg/ml in dimethyl formamide |
| Chemical Name | rel-N-[(1R,2R)-2-hydroxy-1-(4-morpholinylmethyl)-2-phenylethyl]-hexadecanamide, monohydrochloride |
| SDF | Download SDF |
| Canonical SMILES | O=C(CCCCCCCCCCCCCCC)N[C@H](CN1CCOCC1)[C@H](O)C2=CC=CC=C2.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
