D-Luciferin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
D-luciferin是一个细胞渗透性的化学发光的荧光素酶底物，Km值大约为2 μM。在ATP存在下，D-luciferin可以激发氧化脱羧发光。D-luciferin提供了在体和体外胞内检测ATP水平的生物发光信号。D-luciferin可用于测定与感兴趣的启动子相连的萤光素酶基因的表达。D-luciferin和荧光素酶可用于评估细胞内或生物化学测定中ATP的有效性。D-luciferin可以通过静脉内或腹膜内给药。体内和体外生物发光成像（BLI）是用于非侵入性肿瘤成像的重要技术。静脉注射和腹膜内重复性系数分别为80.2％和95.0％。 IP的PEMAX比IV增加5.6倍。与IP相比，静脉给药具有较好的可重复性和更高的灵敏度。这将有利于评估小肿瘤的精确肿瘤负荷，而对于较大的肿瘤没有效果[1]。
参考文献：
Keyaerts M, Verschueren J, Bos T J, et al. Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D-luciferin: effect on intensity, time kinetics and repeatability of photon emission[J]. European journal of nuclear medicine and molecular imaging, 2008, 35(5): 999-1007.
- 1. Tao Xie, Du-Jiang Fu, et al. "CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling." Mol Cancer. 2022 Sep 1;21(1):173. PMID: 36045408
- 2. Libin Zou, Wenbin Chen, et al. "N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability." Cell Death Discov. 2022 Apr 9;8(1):184. PMID: 35397614
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 280.32 |
| Cas No. | 2591-17-5 |
| Formula | C11H8N2O3S2 |
| Solubility | ≥28 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | (S)-2-(6-hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid |
| SDF | Download SDF |
| Canonical SMILES | OC1=CC(S2)=C(N=C2C3=N[C@](C(O)=O)([H])CS3)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
相关生物数据
