β-Galactosidase in Situ Staining Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
β-半乳糖苷酶原位染色试剂盒(β-Galactosidase in Situ Staining Kit)是一种以X-gal为底物检测细胞或组织中β-半乳糖苷酶的试剂盒。其检测的原理是X-gal在β-半乳糖苷酶的催化下可以产生蓝色的产物,通过普通光学显微镜即可检测β-半乳糖苷酶的表达。
本试剂盒经过优化,可以兼容普通细胞培养孔板,移液管等聚苯乙烯材质的耗材或者容器,可以有效地避免由于耗材或容器不兼容所引起的染色效果不佳。并且本试剂盒配制的工作液在使用中不会产生沉淀,更加方便。
图1:Hela细胞转染β-半乳糖苷酶质粒后用本试剂盒检测效果图
Components |
K2182-100 T |
Fixative Solution |
100 mL |
X-gal Solution |
5 mL |
Staining Solution A |
1 mL |
Staining Solution B |
1 mL |
Staining Solution C |
100 mL |
Store the kit at -20°C, stable for 1 year. X-gal Solution should be stored at -20°C away from light. |