Cy7 maleimide (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. Labeled proteins thus obtained are used in NIR bioimaging applications. In tissues or in live organism, NIR imaging systems can be used to visualize distribution of labeled proteins. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In splenocytes, for ease of detection, P22 particles were labeled internally with Cy7-maleimide. P22-Cy7 alone or P22-Cy7 decorated with DecSelf was incubated with splenocytes at 37℃ to encourage phagocytosis [1].
Reference:
[1]. Schwarz, B.; Madden, P.; Avera, J.; Gordon, B.; Larson, K.; Miettinen, H.; Uchida, M.; LaFrance, B.; Basu, G.; Rynda-Apple, A.; Douglas, T. Symmetry Controlled, Genetic Presentation of Bio-Active Proteins on the P22 Virus-Like Particle Using an External Decoration Protein. ACS Nano, 2015, 9(9), 9134–9147.
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
M.Wt | 707.34 |
Formula | C43H51ClN4O3 |
Solubility | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
Chemical Name | 6-[(2E)-3,3-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,3,3-trimethylindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide |
SDF | Download SDF |
Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC(=CC=C4C(C5=CC=CC=C5N4CCCCCC(=O)NCCN6C(=O)C=CC6=O)(C)C)CCC3)C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 750 | 最大发射光波 (nm) | 773 |
消光系数 (M-1cm-1) | 199000 | 量子产量 | 0.3 |
CF260 | 0.022 | CF280 | 0.029 |
质量控制和MSDS
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