Cy7.5 carboxylic acid (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy7.5 carboxylic acid is a free un-activated NIR dye which has low aqueous solubility. As for coupling and labeling reactions, pre-activated Cy7 NHS ester or water-soluble sulfo-Cy7 NHS ester would be considered. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In mice, near-infrared labeling of the materials allowed for non-invasive imaging of the injected hydrogel. Cy7.5 carboxylic acid labeled images can demonstrate localization of the injected hydrogel to the affected kidney. Moreover, the methodology allowed direct quantification of material degradation in vivo [1].
Reference:
[1]. Rodell, C.B.; Rai, R.; Faubel, S.; Burdick, J.A.; Soranno, D.E. Local Immunotherapy via Delivery of Interleukin-10 and Transforming Growth Factor β Antagonist for Treatment of Chronic Kidney Disease. Journal of Controlled Release, 2015, 206, 131–139.
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light |
M.Wt | 685.34 |
Formula | C45H49ClN2O2 |
Solubility | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
Chemical Name | 6-[(2E)-1,1-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]benzo[e]indol-3-yl]hexanoic acid |
SDF | Download SDF |
Canonical SMILES | CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC4=CC(=CC=C5C(C6=C(N5CCCCCC(=O)O)C=CC7=CC=CC=C76)(C)C)CCC4)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 788 | 最大发射光波 (nm) | 808 |
消光系数 (M-1cm-1) | 22300 | 量子产量 | N/A |
CF260 | N/A | CF280 | N/A |