Cy3 Bis NHS ester

Cy3 Bis NHS ester is a reactive fluorescent dye for general labeling of protein amine groups. Cy3 Bis NHS ester includes two reactive groups on one dye molecule. The N-hydroxysuccinimde (NHS) ester is the most popular amine reactive group, used for capturing the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Appling NHS chemistry allows one to conjugate a variety of fluorescent probes (e.g. Cy3, a traditional orange-fluorescent label which is highly water soluble) to primary amines. Thus, target biological compounds can be visualized by Cy3 fluorescence (~550 nm excitation, ~570 nm emission) monitored by various fluorometers, imagers, and microscopes.

References:

1. Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization. Methods in Enzymology, 2014, 536: 87-94.

2. Dyla M, Andersen JL, Kjaergaard M, et al. Engineering a Prototypic P-type ATPase Listeria monocytogenes Ca²⁺-ATPase 1 for Single-Molecule FRET Studies. Bioconjugate Chemistry, 2016, 27(9): 2176-2187.