CBHA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CBHA is a potent and selective inhibitor with ID50 values of 0.01 and 0.07 μM for HDAC1 and HDAC3, respectively [1].
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from lysine amino acid on histone, allowing the histones to wrap the DNA more tightly. HDAC inhibitors hyperacetylate histones and increase transcriptional activity in target genes. HDAC inhibitors have both apoptotic and differentiating effects on various tumor cells [2].
M-carboxycinnamic acid bishydroxamide (CBHA) is a potent and selective HDAC inhibitor. CBHA is a hybrid polar compound that is synthesized to induce terminal differentiation and/or apoptosis in various transformed cells. In MEL cells, CBHA caused accumulation of acetylated histone H4 [1]. In neuroblastoma cell lines, CBHA induced acetylated histone H3 and H4 accumulation. CBHA also led to extensive cell death with LD50 ranged between 1 μM and 4 μM, and induced apoptosis [2].
In human neuroblastoma xenograft SCID mice, CBHA (100 mg/kg and 200 mg/kg) resulted in reductions of average final tumor volume of ~50% and 75%, respectively [3].
References:
[1]. Richon VM1, Emiliani S, Verdin E, et al. A class of hybrid polar inducers of transformed cell differentiation inhibits histone deacetylases. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3003-7.
[2]. Glick RD1, Swendeman SL, Coffey DC, et al. Hybrid polar histone deacetylase inhibitor induces apoptosis and CD95/CD95 ligand expression in human neuroblastoma. Cancer Res. 1999 Sep 1;59(17):4392-9.
[3]. Coffey DC, Kutko MC, Glick RD, et al. The histone deacetylase inhibitor, CBHA, inhibits growth of human neuroblastoma xenografts in vivo, alone and synergistically with all-trans retinoic acid. Cancer Res. 2001 May 1;61(9):3591-4.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 222.2 |
Cas No. | 174664-65-4 |
Formula | C10H10N2O4 |
Synonyms | Histone Deacetylase Inhibitor II,m-Carboxycinnamic Acid bis-Hydroxamide |
Solubility | ≤20mg/ml in DMSO;20mg/ml in dimethyl formamide |
Chemical Name | N-hydroxy-3-[3-(hydroxyamino)-3-oxo-1-propen-1-yl]-benzamide |
SDF | Download SDF |
Canonical SMILES | ONC(=O)\C=C/c1cccc(c1)C(=O)NO |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |