BYK 49187
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BYK 49187是PARP-1和PARP-2的有效抑制剂，针对无细胞重组PARP-1和小鼠PARP-2的pIC50值分别为8.36和7.50[1]。
聚(ADP-核糖) 聚合酶 (PARP)是一个蛋白家族，参与和DNA修复及程序性细胞死亡相关的许多细胞内过程[1]。
BYK 49187是人PARP的有效抑制剂，对PARP1/2没有选择性[1]。在A549、C4I和H9c2细胞中，BYK 49187抑制PAR的形成，pIC50值分别为7.80、7.02和7.65。在体外无细胞和细胞实验中，BYK 49187强效抑制人PARP-1 [1]。
检测大鼠中BYK 49187对心肌梗死面积的影响，低剂量静脉给药几乎没有效果，梗死面积仅减少6％，然而，与对照组相比，更高剂量（先使用3 mg/kg，之后3 mg/kg/h）可显著减少梗死面积（22％）。3 mg/kg静脉给药大鼠的血液样品中，与假手术组相比，BYK49187显著抑制80％的PARP-1[1]。
参考文献：
[1]. Eltze T, Boer R, Wagner T, et al. Imidazoquinolinone, Imidazopyridine, and Isoquinolindione Derivatives as Novel and Potent Inhibitors of the Poly(ADP-ribose) Polymerase (PARP): A Comparison with Standard PARP Inhibitors. Mol Pharmacol, 2008, 74 (6):1587–1598.
| Physical Appearance | Beige solid |
| Storage | Store at 4°C |
| M.Wt | 335.4 |
| Cas No. | 163120-31-8 |
| Formula | C19H21N5O |
| Solubility | <16.77mg/ml in ethanol;insoluble in DMSO |
| Chemical Name | 2-(4-(4-methyl-1H-imidazol-5-yl)piperidin-1-yl)-4H-imidazo[4,5,1-ij]quinolin-6(5H)-one |
| SDF | Download SDF |
| Canonical SMILES | O=C1CCN2C3=C1C=CC=C3N=C2N4CCC(C5=C(C)N=CN5)CC4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
