AMG-47A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AMG-47A是KRAS癌蛋白的去稳定剂.
Ras是一个小GTP酶,参与多种细胞信号途径,包括生长\存活和运动.人类中主要含有三种Ras基因:KRAS\HRAS和NRAS.在人类癌症中已经确定所有三个Ras家族成员的致癌突变.其中,KRAS是所有类型癌症中最频繁突变的原癌基因之一[1].
在HeLa EGFP-KRAS G12V细胞中, AMG-47a治疗48小时减少了30-40％的EGFP信号,处理细胞3至5天显示类似的结果.AMG-47a选择性地影响EGFP-KRAS G12V蛋白水平.三天后,AMG-47a对EGFP-KRAS G12V蛋白水平有轻微影响.AMG-47a处理后,EGFP-KRAS G12V的信号缺失高达50％[1].
参考文献：
1. Carver J, Dexheimer TS, Hsu D et al. A high-throughput assay for small molecule destabilizers of the KRASoncoprotein. PLoS One. 2014 Aug 5;9(8):e103836
| Storage | Store at -20°C |
| M.Wt | 535.56 |
| Cas No. | 882663-88-9 |
| Formula | C29H28F3N5O2 |
| Solubility | Soluble in DMSO |
| Chemical Name | (Z)-4-methyl-3-((E)-2-((2-morpholinoethyl)imino)-2,3-dihydroquinazolin-6-yl)-N-(3-(trifluoromethyl)phenyl)benzimidic acid |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C(C=CC2=N/3)=CC2=CNC3=N/CCN4CCOCC4)C=C(/C(O)=N/C5=CC=CC(C(F)(F)F)=C5)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
