AM 404
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AM 404是一种选择性的大麻素转运抑制剂[1].此外,AM 404是一种CB1大麻素受体和电位香草酸亚型1(TRPV1)激动剂.
内源性大麻素转运体(eCBT)是一种内源性大麻素的转运体.大麻素转运阻断具有镇痛作用.
AM 404是一种选择性的大麻素转运抑制剂.AM404抑制大麻素转运,在神经元和星形胶质细胞中的IC50值分别为1和5 μM.然而,AM404对FAAH活性及花生四烯酸或乙醇胺的摄取没有影响.此外,AM404对CB1受体表现出亲和力,Ki值为1.8 μM[1].在使用苯肾上腺素收缩的大鼠肝动脉中,AM404浓度依赖性地诱发松弛,这被香草酸受体拮抗剂capsazepine抑制.这些结果表明,AM404活化香草酸受体[2].在SK-N-SH神经母细胞瘤中,AM404抑制NFAT和NF-κB信号传导途径[3].
在小鼠中,AM404(10 mg/kg)显著延长和增强大麻素诱导的镇痛作用[1].在大鼠中,AM404能抑制D2家族受体激动剂喹吡罗引起的运动行为.在幼年自发性高血压大鼠中,AM404抑制多动症[4].
参考文献:
[1]. Beltramo M, Stella N, Calignano A, et al. Functional role of high-affinity anandamide transport, as revealed by selective inhibition. Science, 1997, 277(5329): 1094-1097.
[2]. Zygmunt PM, Chuang H, Movahed P, et al. The anandamide transport inhibitor AM404 activates vanilloid receptors. Eur J Pharmacol, 2000, 396(1): 39-42.
[3]. Caballero FJ, Soler-Torronteras R, Lara-Chica M, et al. AM404 inhibits NFAT and NF-κB signaling pathways and impairs migration and invasiveness of neuroblastoma cells. Eur J Pharmacol, 2015, 746: 221-232.
[4]. Beltramo M, de Fonseca FR, Navarro M, et al. Reversal of dopamine D(2) receptor responses by an anandamide transport inhibitor. J Neurosci, 2000, 20(9): 3401-3407.
Physical Appearance | White Waxy solid |
Storage | Store at -20°C |
M.Wt | 395.58 |
Cas No. | 198022-70-7 |
Formula | C26H37NO2 |
Solubility | <19.78mg/ml in ethanol; <19.78mg/ml in DMSO |
Chemical Name | (5Z,8Z,11Z,14Z)-N-(4-hydroxyphenyl)icosa-5,8,11,14-tetraenamide |
SDF | Download SDF |
Canonical SMILES | O=C(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)NC(C=C1)=CC=C1O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |