Alternariol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alternariol (AOH) is a toxic metabolite produced by Alternaria alternata and A. tenuissima, which grow on corn, rice, fruits, vegetables and oilseed. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff [1].
Alternariol (0.8 μM) inhibited P4 secretion. Alternariol (1.6 μM) significantly reduced cell viability [1]. Alternariol (0.8 μM) reduced the expression of P450scc protein. Alternariol (1.6 μM) significantly reduced the abundance of α-tubulin and also clearly affected actin protein concentrations. Alternariol specifically inhibited P4 secretion in cultured porcine granulosa cells [1]. Twelve hours of light exposure was sufficient to decrease significantly the production of alternariol [2]. Light inhibited the production of alternariol in Alternaria alternate [2]. Alternariol was preferentially metabolized by cytochrome P450 (CYP) 1A1 and 1A2. Alternariol enhanced the levels of CYP1A1 in murine hepatoma cells (Hepa-1c1c7) but not in cells with inactivated AhR (Hepa-1c1c12) or ARNT (Hepa-1c1c4). Alternariol showed no effects on the production of reactive oxygen species but reduced cell counts in Hepa-1c1c7 cells after 24 and 48 h. At 48 h, AOH increased apoptosis dependent on AhR and ARNT [3].
References:
[1] Tiemann U, Tomek W, Schneider F, et al. The mycotoxins alternariol and alternariol methyl ether negatively affect progesterone synthesis in porcine granulosa cells in vitro[J]. Toxicology Letters, 2009, 186(2): 139-145.
[2] Sderhll K, Svensson E, Unestam T. Light inhibits the production of alternariol and alternariol monomethyl ether in Alternaria alternata[J]. Applied and environmental microbiology, 1978, 36(5): 655-657.
[3] Schreck I, Deigendesch U, Burkhardt B, et al. The Alternaria mycotoxins alternariol and alternariol methyl ether induce cytochrome P450 1A1 and apoptosis in murine hepatoma cells dependent on the aryl hydrocarbon receptor[J]. Archives of toxicology, 2012, 86(4): 625-632.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 258.2 |
Cas No. | 641-38-3 |
Formula | C14H10O5 |
Synonyms | AOH,NSC 638263 |
Solubility | ≤0.5mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide |
Chemical Name | 3,7,9-trihydroxy-1-methyl-6H-dibenzo[b,d]pyran-6-one |
SDF | Download SDF |
Canonical SMILES | CC1=C(C2=C(C(O3)=O)C(O)=CC(O)=C2)C3=CC(O)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |