VEGFR-2, human recombinant protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Vascular endothelial growth factor receptor 2 (VEGFR-2) belongs to the family of receptor tyrosine kinases (RTKs) and is almost exclusively restricted to endothelial cells. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. VEGFR-2 has seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 mediates mitogenic activity in endothelial cells, which leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
Recombinant human VEGFR-2 is produced in baculovirus as a monomeric, glycosylated, polypeptide and having a total molecular mass of 116 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for ligand binding. The VEGFR-2 is purified by proprietary chromatographic techniques.
| Determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors. | ||||||
| ED50 | 10.0 ng/ml. | |||||
| Specific activity | 100,000IU/mg. | |||||
Quality Control & DataSheet
- View current batch:
-
Purity: ≥95%, by SDS-PAGE.
Endotoxin Level:<0.1 ng/μg
| Gene ID: | 3791 | Accession #: | P35968 |
| Alternate Names: | VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1. | ||
| Source: | Insect cells. | M.Wt: | 116 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20℃ |
| Formulation: | Lyophilized from a sterile solution containing 25 mM MES pH 5.5 and 100 mM NaCl. | ||
| Solubility: | Reconstitute in sterile water to a concentration not less than 0.1 mg/ml. This solution can then be stored at 4℃ for 2-7 days. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) then store at -20℃. Avoid freeze-thaw cycles. | ||
| Shipping Condition: | Blue ice. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||