IFN-gamma, murine recombinant
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with rat IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.
Reference:
1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol
2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55
3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56
4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.
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Gene ID |
15978 |
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Accession # |
P01580 |
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Alternate Names |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
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AA Sequence |
HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 µm filtered solution in 20 mM Tris-HCl pH8.0, 300 mM NaCl, containing 5 % trehalose, 0.05 % Tween-20. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled 20 mM Tris-HCl pH8.0 with 50 mM NaCl to a concentration of 0.1-0.3 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 106 IU/mg. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
- 1. Yuanping Li, Dan Zheng, et al. "Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization." J Immunol Res. 2020 Oct 10;2020:2462039. PMID: 33102606
- 2. Xiao-yan Zhou, Rong Gao, et al. "Trained Innate Immunity by Repeated Low-Dose Lipopolysaccharide Injections Displays Long-Term Neuroprotective Effects." Mediators Inflamm. 2020 Oct 1;2020:8191079. PMID: 33061831
质量控制和DataSheet
- 批次:
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纯度 > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/µg of rMuIFN-γ as determined by LAL method.
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