TNF-alpha, recombinant rat protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Rat tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a transmembrane protein with 235 aa that is cleaved to the soluble monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, viral replication, tumorigenesis, apoptosis, cell proliferation, differentiation, coagulation and lipid metabolism. TNF-α is mainly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells.
| Determined by the cytolysis of murine L929 cells in the presence of actinomycin D. | ||||||
| ED50 | < 0.05 ng/ml. | |||||
| Specific activity | > 2*107 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥95%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
相关生物数据
相关生物数据
| Gene ID: | 24835 | Accession #: | P16599.1 |
| Alternate Names: | TNFA, TNF alpha; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha. | ||
| Source: | Escherichia Coli. | M.Wt: | 17 kDa |
| Appearance: | Lyophilized powder | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized from 1× PBS. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||