T4 DNA Ligase (RNase-free)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T4 DNA Ligase是一种分子量为55.3kDa的单链多肽，可催化双链DNA或RNA上相邻的5’磷酸和3’羟基末端形成磷酸二酯键，但对单链核酸无催化活性，其催化反应需ATP作为辅助因子。
T4 DNA连接酶适应性广，不仅可以将DNA片段与黏性末端或平末端结合，还可以修补双链DNA、双链RNA或DNA/RNA杂合体上的单链缺口，常用于DNA片段和载体、linker或adaptor的连接，缺口修复，以及线性 DNA 自环化等。
| Components | 40 KU | 100 KU | 200 KU |
| T4 DNA Ligase (400 U/μL) | 0.1 mL | 0.25 mL | 0.5 mL |
| 10X T4 DNA Ligase Reaction Buffer | 1 mL | 2.5 mL | 5 mL |
Store the components at -20 °C. | |||