Recombinant Murine Vascular Endothelial Growth Factor 164
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
血管内皮生长因子是细胞产生的生长因子的一个亚家族，可刺激血管生成和血管生成。VEGF的正常功能是在胚胎发育过程中产生新的血管，在受伤后产生新的血管，在运动后产生肌肉，以及建立新的血管（侧支循环）以绕过阻塞的血管。小鼠和大鼠表达长度为 120、164 和 188 个氨基酸 （a.a.） 的交替剪接亚型。重组小鼠VEGF165含有 165 个氨基酸残基（在 N 末端有一个 met），它是一种二硫键连接的同源二聚体。此外，它与大鼠的相应区域分别具有97%的a.a.序列同一性，与人和猪的序列一致性为89%，与牛的相似性为88%，与猫、马和犬VEGF的相似性为90%。
Reference
1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9
2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94
3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.
| Accession # | Q00731 |
| Alternate Names | Vascular Endothelial Growth Factor Isoform 164 |
| Source | Escherichia coli. |
| M.Wt | Approximately 38.8 kDa, a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains with Met at N-terminus. |
| AA Sequence | MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
-
纯度 > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rMuVEGF164 as determined by LAL method.