Recombinant Murine Growth Differentiation Factor 5/Bone Morphogenetic Protein-14
Catalog No.
P1409
重组鼠生长分化因子5/骨形态发生蛋白-14
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生长/分化因子（GDF-1 至 GDF-15）是 TGF-β 超家族蛋白 BMP 家族的成员。它们以无活性的前原蛋白形式产生，然后被切割并组装成活性分泌的同型二聚体。GDF二聚体是二硫键连接的，但GDF-3和-9除外。GDF蛋白在胚胎发育过程中很重要，特别是在骨骼、神经和肌肉系统中。
| Gene ID | 14563 |
| Accession # | P43027 |
| Source | Escherichia coli. |
| M.Wt | Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids. |
| AA Sequence | APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
-
纯度 > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/μg of rMuGDF-5/BMP-14 as determined by LAL method.