Recombinant Murine Dendritic Cell Inflammatory Protein-1/CXCL3
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CXCL3在小鼠中也称为DCIP1，在大鼠中称为CINC2，在人类中称为Groγ，属于CxC趋化因子家族。该基因在小鼠体内由CXCL3基因编码。CXCL3的功能性受体已被鉴定为CXCR2。与其他GRO蛋白类似，CXCL3是一种有效的中性粒细胞引诱剂和激活剂。CXCL3在炎症中发挥作用，并以自分泌的方式作用于内皮细胞。小鼠CXCL3的氨基酸序列与人CXCL3有57%的。
Reference
1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9
2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93
3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83
4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84
5. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
| Gene ID | 330122 |
| Accession # | Q6W5C0 |
| Source | Escherichia coli. |
| M.Wt | Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids. |
| AA Sequence | AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rMuDCIP-1/CXCL3 as determined by LAL method.