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Recombinant Mouse M-CSF

 
Catalog No.
P1243
重组小鼠巨噬细胞集落刺激因子
组合的产品项目
规格价格库存 数量
10ug
¥ 2,216.00
Ship with 5-10 days
100ug
¥ 10,487.00
Ship with 5-10 days
500ug
¥ 23,530.00
Ship with 5-10 days

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A

背景

Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229 a.a. of mature mouse M-CSF shares 87 %, 83 %, 82 % and 81 % sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. 

Reference:

1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.

2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.

3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.

4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.

Description

Gene ID

 

Accession #

Q3U4F9

Alternate Names

CSF-1, MGI-IM

Source

Escherichia coli.

M.Wt

Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.

AA Sequence

KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

 

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

质量控制

质量控制和DataSheet

批次:
    纯度 > 95 % by SDS-PAGE and HPLC analyses.
    Endotoxin: Less than 1 EU/μg of rMuM-CSF as determined by LAL method.
  • Datasheet