Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
人TNF相关细胞凋亡受体弱诱导剂（TWEAKR）又称肿瘤坏死因子受体超家族成员12A前体（基因名称TNFRSF12A）或成纤维细胞生长因子诱导的14 kD蛋白，与TNFR家族有远亲关系，在细胞外区域包含一个富含半胱氨酸的结构域和一个TNFR相关因子结合结构域，但不包含死亡结构域（DD）细胞质区域。它在脾脏、胸腺、外周血淋巴细胞、结肠和小肠中表达。TWEAK 受体的信号转导可以通过膜锚定或可溶性 TWEAK 激活。此外，它还在TWEAK诱导的内皮细胞迁移、增殖和血管生成中发挥作用。人和小鼠TWEAK R共享82%的a.a.序列同一性。
Reference
1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86
2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51
3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33
4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.
| Gene ID | 51330 |
| Accession # | Q9NP84 |
| Alternate Names | TNFRSF12A, FGF-inducible 14, CD266 |
| Source | Escherichia coli. |
| M.Wt | Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids. |
| AA Sequence | EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuTWEAK Receptor as determined by LAL method.