Recombinant Human R-Spondin 1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
R-Spondin 1(RSPO1),也被称为富含半胱氨酸和单一含血小板反应蛋白结构域的蛋白3(Cristin 3),是一种27kDa的分泌蛋白,与其他三个R-Spondi家族成员[1,2]共享约40%的氨基酸(aa)同一性。所有R-海绵蛋白调节Wnt/β-儿茶素信号传导,但具有不同的表达模式[1-3]。人R-Spondin 1(aa21-263)与小鼠、大鼠、马、狗、山羊和奶牛RSPO-1的氨基酸同源性分别为89%、87%、92%、91%、91%和89%。R-Spondin 1与Wnt拮抗剂DKK-1竞争与Wnt共受体Kreman和LRP-6的结合,减少其DKK-1介导的内化[4]。然而,关于R-Spondin 1是否直接结合LRP-6的报道众说纷纭[4-6]。R-Spondin 1在顶板边界的早期发育中表达,并被认为有助于背神经管的发育[3,7]。随着类器官领域的扩大,人们对R-Spondin 1作为细胞培养补充剂的兴趣与日俱增。R-Spondin 1被广泛用于类器官细胞培养工作流程,作为促进3D类器官生长和存活的重要成分[8]。在结构上类似于其他R-Spondin,R-Spondin-1包含两个相邻的富含半胱氨酸的弗林蛋白样结构域(aa 34-135),具有一个潜在的N-糖基化位点,随后是血栓反应蛋白(TSP-1)基序(aa 147-207)和富含碱性残基的区域(aa 211-263)。β-连环蛋白稳定只需要弗林样结构域[2,9]。在C末端的假定的核定位信号可能允许在核中的一些表达[10]。200和236个氨基酸的潜在亚型分别具有交替的、较短的N末端或缺失氨基酸146-208[11]
Reference
[1]. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
[2]. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
[3]. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
[4]. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
[5]. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
[6]. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
[7]. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
[8]. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
[9]. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
[10]. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
Accession # |
Q2MKA7 |
Alternate Names |
Cristin 3; HRspo1; roof plate-specific spondin; RSPO1; RSpondin 1; R-Spondin 1 |
Source |
Human embryonic kidney cell, HEK293-derived human R-Spondin 1 protein |
Protein sequence |
Ser21-Ala263 |
M.Wt |
25.6 kDa |
Appearance |
Solution protein. |
Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
Concentration |
0. 2 mg/mL |
Formulation |
Dissolved in sterile PBS buffer. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
Biological Activity |
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is 1-10 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a. |
Shipping Condition |
Shipping with dry ice. |
Handling |
Centrifuge the vial prior to opening. |
Usage |
For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
-
纯度 > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.